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kb dna ladder  (Thermo Fisher)


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    Structured Review

    Thermo Fisher kb dna ladder
    Kb Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/molecular+weight+marker/DNA+Molecular+Weight+Marker%2C+1Kb+Ladder/pm42347511-158-3-6
    Average 97 stars, based on 1 article reviews
    kb dna ladder - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Mortality associated with Dictyocaulus cervi in farmed red deer ( Cervus elaphus ) in Romania
    Article Snippet: Amplification was carried out in a Thermal Cycler T1000TM (Bio-Rad, London, UK) using the following conditions: initial denaturation at 95 °C for 5 min, followed by 40 cycles of denaturation at 95 °C for 45 s, annealing at 60 °C for 45 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. .. The PCR products were visualized by electrophoresis on a 1.5% agarose gel stained with ECO Safe 20,000× Nucleic Acid Staining Solution (Pacific Image Electronics, New Taipei, Taiwan), and compared to a molecular weight marker (O’GeneRulerTM 100 bp DNA Ladder, Thermo Fisher Scientific Inc., Waltham, MA, USA). .. The PCR product was purified using the ISOLATE II PCR and Gel Kit (Bioline Meridian Bioscience, Luckenwalde, Germany) and submitted for sequencing (Macrogen Europe, Amsterdam, The Netherlands).

    Article Title: Antibiotic-Driven Evolutionary Engineering in Salmonella Heidelberg Reveals Genomic Signatures of Attenuation.
    Article Snippet: .. All PCR products were visualized by loading 9 μL of the product and 1 μL of loading dye onto a 1.5% agarose gel, using a 100 bp DNA ladder (Invitrogen, Carlsbad, CA, USA) as the molecular weight marker and applying 10 V per cm of gel. ..

    Article Title: Occurrence and Genetic Diversity of Trichomonas gallinae in Captive Synanthropic Birds in Southeastern Brazil.
    Article Snippet: .. PCR products were resolved via electrophoresis in a 1.0% agarose gel stained with ethidium bromide (0.5 μL/mL) in TBE buffer (pH 8.0), https://doi.org/10.3390/pathogens15040428 run at 100 V/150 mA for 50 min. A 100 bp molecular weight marker (Life Technologies®, Carlsbad, CA, USA) was used to estimate product size, visualized under a ChemiDoc MP Imaging System (Bio-Rad®, Hercules, CA, USA). ..

    Article Title: Integrative analysis of toxin-antitoxin systems, genomics, and phenotypic traits reveal phylogroup-dependent diversity among ExPEC bloodstream isolates.
    Article Snippet: Amplifications were performed using a VeritiTM 96-Well Thermal Cycler (Applied Biosystems). .. Following amplification, 5 μL of each PCR product was analyzed by electrophoresis on a 1.0% agarose gel stained with GelRed (Biotium, USA) in 1 × Tris-acetate-EDTA (TAE) buffer (40 mM Tris-acetate; 2 mM EDTA), at a constant voltage of 80 V. The 1 Kb Plus DNA Ladder (Invitrogen) was used as the molecular weight marker. .. Gels were visualized and documented using the Alliance 6.7 imaging system (UVItec Cambridge, UK).

    Article Title: Molecular characterization of a naturally occurring isolate of Begomovirus solanumkarnatakaense causing breakdown of Ty-3-mediated resistance in tomato plants with increased disease severity under elevated temperature.
    Article Snippet: .. M: Molecular weight marker (GeneRulerTM 100 bp plus DNA ladder, Thermo Fisher Scientific, USA); +Ve: Positive control with known plasmid DNA; H: Negative control using TNA from healthy plant; -Ve: PCR reagent control; Lane 1 to 18: PCR amplified product obtained using TNAs from 18 tomato genotypes as template in the order: ToLCD Tol-1, ToLCD Tol -2, ToLCD Tol -3, ToLCD Tol -4, ToLCD Tol -5, ToLCD Tol -6, ToLCD Tol -7, ToLCD Tol -8, ToLCD Tol -9, ToLCD Tol -10, ToLCD Tol -11, Pusa Ruby, ToLCD Tol -12, ToLCD Tol -13, ToLCD Tol -14, ToLCD Tol -15, ToLCD Tol -16, ToLCD Tol - 17. (C) Agarose gel (1%) electrophoresis of BamHI-digested RCA amplified viral DNA (lane 1). .. M: Molecular weight marker (GeneRulerTM 1 kb plus DNA ladder, Thermo Fisher Scientific, USA).

    Electrophoresis:

    Article Title: Mortality associated with Dictyocaulus cervi in farmed red deer ( Cervus elaphus ) in Romania
    Article Snippet: Amplification was carried out in a Thermal Cycler T1000TM (Bio-Rad, London, UK) using the following conditions: initial denaturation at 95 °C for 5 min, followed by 40 cycles of denaturation at 95 °C for 45 s, annealing at 60 °C for 45 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. .. The PCR products were visualized by electrophoresis on a 1.5% agarose gel stained with ECO Safe 20,000× Nucleic Acid Staining Solution (Pacific Image Electronics, New Taipei, Taiwan), and compared to a molecular weight marker (O’GeneRulerTM 100 bp DNA Ladder, Thermo Fisher Scientific Inc., Waltham, MA, USA). .. The PCR product was purified using the ISOLATE II PCR and Gel Kit (Bioline Meridian Bioscience, Luckenwalde, Germany) and submitted for sequencing (Macrogen Europe, Amsterdam, The Netherlands).

    Article Title: Occurrence and Genetic Diversity of Trichomonas gallinae in Captive Synanthropic Birds in Southeastern Brazil.
    Article Snippet: .. PCR products were resolved via electrophoresis in a 1.0% agarose gel stained with ethidium bromide (0.5 μL/mL) in TBE buffer (pH 8.0), https://doi.org/10.3390/pathogens15040428 run at 100 V/150 mA for 50 min. A 100 bp molecular weight marker (Life Technologies®, Carlsbad, CA, USA) was used to estimate product size, visualized under a ChemiDoc MP Imaging System (Bio-Rad®, Hercules, CA, USA). ..

    Article Title: Integrative analysis of toxin-antitoxin systems, genomics, and phenotypic traits reveal phylogroup-dependent diversity among ExPEC bloodstream isolates.
    Article Snippet: Amplifications were performed using a VeritiTM 96-Well Thermal Cycler (Applied Biosystems). .. Following amplification, 5 μL of each PCR product was analyzed by electrophoresis on a 1.0% agarose gel stained with GelRed (Biotium, USA) in 1 × Tris-acetate-EDTA (TAE) buffer (40 mM Tris-acetate; 2 mM EDTA), at a constant voltage of 80 V. The 1 Kb Plus DNA Ladder (Invitrogen) was used as the molecular weight marker. .. Gels were visualized and documented using the Alliance 6.7 imaging system (UVItec Cambridge, UK).

    Article Title: Molecular characterization of a naturally occurring isolate of Begomovirus solanumkarnatakaense causing breakdown of Ty-3-mediated resistance in tomato plants with increased disease severity under elevated temperature.
    Article Snippet: .. M: Molecular weight marker (GeneRulerTM 100 bp plus DNA ladder, Thermo Fisher Scientific, USA); +Ve: Positive control with known plasmid DNA; H: Negative control using TNA from healthy plant; -Ve: PCR reagent control; Lane 1 to 18: PCR amplified product obtained using TNAs from 18 tomato genotypes as template in the order: ToLCD Tol-1, ToLCD Tol -2, ToLCD Tol -3, ToLCD Tol -4, ToLCD Tol -5, ToLCD Tol -6, ToLCD Tol -7, ToLCD Tol -8, ToLCD Tol -9, ToLCD Tol -10, ToLCD Tol -11, Pusa Ruby, ToLCD Tol -12, ToLCD Tol -13, ToLCD Tol -14, ToLCD Tol -15, ToLCD Tol -16, ToLCD Tol - 17. (C) Agarose gel (1%) electrophoresis of BamHI-digested RCA amplified viral DNA (lane 1). .. M: Molecular weight marker (GeneRulerTM 1 kb plus DNA ladder, Thermo Fisher Scientific, USA).

    Agarose Gel Electrophoresis:

    Article Title: Mortality associated with Dictyocaulus cervi in farmed red deer ( Cervus elaphus ) in Romania
    Article Snippet: Amplification was carried out in a Thermal Cycler T1000TM (Bio-Rad, London, UK) using the following conditions: initial denaturation at 95 °C for 5 min, followed by 40 cycles of denaturation at 95 °C for 45 s, annealing at 60 °C for 45 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. .. The PCR products were visualized by electrophoresis on a 1.5% agarose gel stained with ECO Safe 20,000× Nucleic Acid Staining Solution (Pacific Image Electronics, New Taipei, Taiwan), and compared to a molecular weight marker (O’GeneRulerTM 100 bp DNA Ladder, Thermo Fisher Scientific Inc., Waltham, MA, USA). .. The PCR product was purified using the ISOLATE II PCR and Gel Kit (Bioline Meridian Bioscience, Luckenwalde, Germany) and submitted for sequencing (Macrogen Europe, Amsterdam, The Netherlands).

    Article Title: Antibiotic-Driven Evolutionary Engineering in Salmonella Heidelberg Reveals Genomic Signatures of Attenuation.
    Article Snippet: .. All PCR products were visualized by loading 9 μL of the product and 1 μL of loading dye onto a 1.5% agarose gel, using a 100 bp DNA ladder (Invitrogen, Carlsbad, CA, USA) as the molecular weight marker and applying 10 V per cm of gel. ..

    Article Title: Occurrence and Genetic Diversity of Trichomonas gallinae in Captive Synanthropic Birds in Southeastern Brazil.
    Article Snippet: .. PCR products were resolved via electrophoresis in a 1.0% agarose gel stained with ethidium bromide (0.5 μL/mL) in TBE buffer (pH 8.0), https://doi.org/10.3390/pathogens15040428 run at 100 V/150 mA for 50 min. A 100 bp molecular weight marker (Life Technologies®, Carlsbad, CA, USA) was used to estimate product size, visualized under a ChemiDoc MP Imaging System (Bio-Rad®, Hercules, CA, USA). ..

    Article Title: Trypanosoma minasense Parasitism in Free-Living and Captive Invasive Marmosets (Callitrichidae, Primates) in Seropédica, Rio de Janeiro, Brazil.
    Article Snippet: .. The quantity and quality of the extracted DNA were estimated on a 0.8% agarose gel using Lambda DNA (Promega®) as a molecular weight marker and via NanoDrop 2000 (Thermo Fisher Scientific®). ..

    Article Title: Integrative analysis of toxin-antitoxin systems, genomics, and phenotypic traits reveal phylogroup-dependent diversity among ExPEC bloodstream isolates.
    Article Snippet: Amplifications were performed using a VeritiTM 96-Well Thermal Cycler (Applied Biosystems). .. Following amplification, 5 μL of each PCR product was analyzed by electrophoresis on a 1.0% agarose gel stained with GelRed (Biotium, USA) in 1 × Tris-acetate-EDTA (TAE) buffer (40 mM Tris-acetate; 2 mM EDTA), at a constant voltage of 80 V. The 1 Kb Plus DNA Ladder (Invitrogen) was used as the molecular weight marker. .. Gels were visualized and documented using the Alliance 6.7 imaging system (UVItec Cambridge, UK).

    Article Title: Molecular characterization of a naturally occurring isolate of Begomovirus solanumkarnatakaense causing breakdown of Ty-3-mediated resistance in tomato plants with increased disease severity under elevated temperature.
    Article Snippet: .. M: Molecular weight marker (GeneRulerTM 100 bp plus DNA ladder, Thermo Fisher Scientific, USA); +Ve: Positive control with known plasmid DNA; H: Negative control using TNA from healthy plant; -Ve: PCR reagent control; Lane 1 to 18: PCR amplified product obtained using TNAs from 18 tomato genotypes as template in the order: ToLCD Tol-1, ToLCD Tol -2, ToLCD Tol -3, ToLCD Tol -4, ToLCD Tol -5, ToLCD Tol -6, ToLCD Tol -7, ToLCD Tol -8, ToLCD Tol -9, ToLCD Tol -10, ToLCD Tol -11, Pusa Ruby, ToLCD Tol -12, ToLCD Tol -13, ToLCD Tol -14, ToLCD Tol -15, ToLCD Tol -16, ToLCD Tol - 17. (C) Agarose gel (1%) electrophoresis of BamHI-digested RCA amplified viral DNA (lane 1). .. M: Molecular weight marker (GeneRulerTM 1 kb plus DNA ladder, Thermo Fisher Scientific, USA).

    Staining:

    Article Title: Mortality associated with Dictyocaulus cervi in farmed red deer ( Cervus elaphus ) in Romania
    Article Snippet: Amplification was carried out in a Thermal Cycler T1000TM (Bio-Rad, London, UK) using the following conditions: initial denaturation at 95 °C for 5 min, followed by 40 cycles of denaturation at 95 °C for 45 s, annealing at 60 °C for 45 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. .. The PCR products were visualized by electrophoresis on a 1.5% agarose gel stained with ECO Safe 20,000× Nucleic Acid Staining Solution (Pacific Image Electronics, New Taipei, Taiwan), and compared to a molecular weight marker (O’GeneRulerTM 100 bp DNA Ladder, Thermo Fisher Scientific Inc., Waltham, MA, USA). .. The PCR product was purified using the ISOLATE II PCR and Gel Kit (Bioline Meridian Bioscience, Luckenwalde, Germany) and submitted for sequencing (Macrogen Europe, Amsterdam, The Netherlands).

    Article Title: A bifunctional CPG2-anti PD-L1 fusion protein combining specific tumor targeting and enzyme-mediated prodrug activation.
    Article Snippet: The specific targeting of therapeutic agents to tumor cells remains a pivotal challenge in cancer treatment.. While antibody-directed enzyme prodrug therapy (ADEPT) offers a promising approach by delivering enzymes such as carboxypeptidase G2 (CPG2) to the tumor microenvironment, achieving efficient targeting and multifunctionality remains a significant hurdle.. This study developed a novel bifunctional fusion protein by integrating an anti-PD-L1 Jo ur na l P re -p ro of Journal Pre-proof 2 nanobody with CPG2, enabling specific immune checkpoint targeting, and localized prodrug activation.

    Article Title: Occurrence and Genetic Diversity of Trichomonas gallinae in Captive Synanthropic Birds in Southeastern Brazil.
    Article Snippet: .. PCR products were resolved via electrophoresis in a 1.0% agarose gel stained with ethidium bromide (0.5 μL/mL) in TBE buffer (pH 8.0), https://doi.org/10.3390/pathogens15040428 run at 100 V/150 mA for 50 min. A 100 bp molecular weight marker (Life Technologies®, Carlsbad, CA, USA) was used to estimate product size, visualized under a ChemiDoc MP Imaging System (Bio-Rad®, Hercules, CA, USA). ..

    Article Title: Integrative analysis of toxin-antitoxin systems, genomics, and phenotypic traits reveal phylogroup-dependent diversity among ExPEC bloodstream isolates.
    Article Snippet: Amplifications were performed using a VeritiTM 96-Well Thermal Cycler (Applied Biosystems). .. Following amplification, 5 μL of each PCR product was analyzed by electrophoresis on a 1.0% agarose gel stained with GelRed (Biotium, USA) in 1 × Tris-acetate-EDTA (TAE) buffer (40 mM Tris-acetate; 2 mM EDTA), at a constant voltage of 80 V. The 1 Kb Plus DNA Ladder (Invitrogen) was used as the molecular weight marker. .. Gels were visualized and documented using the Alliance 6.7 imaging system (UVItec Cambridge, UK).

    Molecular Weight:

    Article Title: Mortality associated with Dictyocaulus cervi in farmed red deer ( Cervus elaphus ) in Romania
    Article Snippet: Amplification was carried out in a Thermal Cycler T1000TM (Bio-Rad, London, UK) using the following conditions: initial denaturation at 95 °C for 5 min, followed by 40 cycles of denaturation at 95 °C for 45 s, annealing at 60 °C for 45 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. .. The PCR products were visualized by electrophoresis on a 1.5% agarose gel stained with ECO Safe 20,000× Nucleic Acid Staining Solution (Pacific Image Electronics, New Taipei, Taiwan), and compared to a molecular weight marker (O’GeneRulerTM 100 bp DNA Ladder, Thermo Fisher Scientific Inc., Waltham, MA, USA). .. The PCR product was purified using the ISOLATE II PCR and Gel Kit (Bioline Meridian Bioscience, Luckenwalde, Germany) and submitted for sequencing (Macrogen Europe, Amsterdam, The Netherlands).

    Article Title: An effective tumor-inhibiting siRNA delivery platform.
    Article Snippet: The siRNA band densities were visualized with an ultraviolet system (ChemiDoc Touch, BIO-RAD, Hercules, CA, USA). .. Molecular weight marker (MW; GeneRuler 100 bp Plus DNA ladder, ThermoFisher Scientific, Waltham, MA). ..

    Article Title: Antibiotic-Driven Evolutionary Engineering in Salmonella Heidelberg Reveals Genomic Signatures of Attenuation.
    Article Snippet: .. All PCR products were visualized by loading 9 μL of the product and 1 μL of loading dye onto a 1.5% agarose gel, using a 100 bp DNA ladder (Invitrogen, Carlsbad, CA, USA) as the molecular weight marker and applying 10 V per cm of gel. ..

    Article Title: A bifunctional CPG2-anti PD-L1 fusion protein combining specific tumor targeting and enzyme-mediated prodrug activation.
    Article Snippet: The specific targeting of therapeutic agents to tumor cells remains a pivotal challenge in cancer treatment.. While antibody-directed enzyme prodrug therapy (ADEPT) offers a promising approach by delivering enzymes such as carboxypeptidase G2 (CPG2) to the tumor microenvironment, achieving efficient targeting and multifunctionality remains a significant hurdle.. This study developed a novel bifunctional fusion protein by integrating an anti-PD-L1 Jo ur na l P re -p ro of Journal Pre-proof 2 nanobody with CPG2, enabling specific immune checkpoint targeting, and localized prodrug activation.

    Article Title: Occurrence and Genetic Diversity of Trichomonas gallinae in Captive Synanthropic Birds in Southeastern Brazil.
    Article Snippet: .. PCR products were resolved via electrophoresis in a 1.0% agarose gel stained with ethidium bromide (0.5 μL/mL) in TBE buffer (pH 8.0), https://doi.org/10.3390/pathogens15040428 run at 100 V/150 mA for 50 min. A 100 bp molecular weight marker (Life Technologies®, Carlsbad, CA, USA) was used to estimate product size, visualized under a ChemiDoc MP Imaging System (Bio-Rad®, Hercules, CA, USA). ..

    Article Title: Trypanosoma minasense Parasitism in Free-Living and Captive Invasive Marmosets (Callitrichidae, Primates) in Seropédica, Rio de Janeiro, Brazil.
    Article Snippet: .. The quantity and quality of the extracted DNA were estimated on a 0.8% agarose gel using Lambda DNA (Promega®) as a molecular weight marker and via NanoDrop 2000 (Thermo Fisher Scientific®). ..

    Article Title: Integrative analysis of toxin-antitoxin systems, genomics, and phenotypic traits reveal phylogroup-dependent diversity among ExPEC bloodstream isolates.
    Article Snippet: Amplifications were performed using a VeritiTM 96-Well Thermal Cycler (Applied Biosystems). .. Following amplification, 5 μL of each PCR product was analyzed by electrophoresis on a 1.0% agarose gel stained with GelRed (Biotium, USA) in 1 × Tris-acetate-EDTA (TAE) buffer (40 mM Tris-acetate; 2 mM EDTA), at a constant voltage of 80 V. The 1 Kb Plus DNA Ladder (Invitrogen) was used as the molecular weight marker. .. Gels were visualized and documented using the Alliance 6.7 imaging system (UVItec Cambridge, UK).

    Article Title: Molecular characterization of a naturally occurring isolate of Begomovirus solanumkarnatakaense causing breakdown of Ty-3-mediated resistance in tomato plants with increased disease severity under elevated temperature.
    Article Snippet: .. M: Molecular weight marker (GeneRulerTM 100 bp plus DNA ladder, Thermo Fisher Scientific, USA); +Ve: Positive control with known plasmid DNA; H: Negative control using TNA from healthy plant; -Ve: PCR reagent control; Lane 1 to 18: PCR amplified product obtained using TNAs from 18 tomato genotypes as template in the order: ToLCD Tol-1, ToLCD Tol -2, ToLCD Tol -3, ToLCD Tol -4, ToLCD Tol -5, ToLCD Tol -6, ToLCD Tol -7, ToLCD Tol -8, ToLCD Tol -9, ToLCD Tol -10, ToLCD Tol -11, Pusa Ruby, ToLCD Tol -12, ToLCD Tol -13, ToLCD Tol -14, ToLCD Tol -15, ToLCD Tol -16, ToLCD Tol - 17. (C) Agarose gel (1%) electrophoresis of BamHI-digested RCA amplified viral DNA (lane 1). .. M: Molecular weight marker (GeneRulerTM 1 kb plus DNA ladder, Thermo Fisher Scientific, USA).

    Marker:

    Article Title: Mortality associated with Dictyocaulus cervi in farmed red deer ( Cervus elaphus ) in Romania
    Article Snippet: Amplification was carried out in a Thermal Cycler T1000TM (Bio-Rad, London, UK) using the following conditions: initial denaturation at 95 °C for 5 min, followed by 40 cycles of denaturation at 95 °C for 45 s, annealing at 60 °C for 45 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 5 min. .. The PCR products were visualized by electrophoresis on a 1.5% agarose gel stained with ECO Safe 20,000× Nucleic Acid Staining Solution (Pacific Image Electronics, New Taipei, Taiwan), and compared to a molecular weight marker (O’GeneRulerTM 100 bp DNA Ladder, Thermo Fisher Scientific Inc., Waltham, MA, USA). .. The PCR product was purified using the ISOLATE II PCR and Gel Kit (Bioline Meridian Bioscience, Luckenwalde, Germany) and submitted for sequencing (Macrogen Europe, Amsterdam, The Netherlands).

    Article Title: An effective tumor-inhibiting siRNA delivery platform.
    Article Snippet: The siRNA band densities were visualized with an ultraviolet system (ChemiDoc Touch, BIO-RAD, Hercules, CA, USA). .. Molecular weight marker (MW; GeneRuler 100 bp Plus DNA ladder, ThermoFisher Scientific, Waltham, MA). ..

    Article Title: Antibiotic-Driven Evolutionary Engineering in Salmonella Heidelberg Reveals Genomic Signatures of Attenuation.
    Article Snippet: .. All PCR products were visualized by loading 9 μL of the product and 1 μL of loading dye onto a 1.5% agarose gel, using a 100 bp DNA ladder (Invitrogen, Carlsbad, CA, USA) as the molecular weight marker and applying 10 V per cm of gel. ..

    Article Title: A bifunctional CPG2-anti PD-L1 fusion protein combining specific tumor targeting and enzyme-mediated prodrug activation.
    Article Snippet: The specific targeting of therapeutic agents to tumor cells remains a pivotal challenge in cancer treatment.. While antibody-directed enzyme prodrug therapy (ADEPT) offers a promising approach by delivering enzymes such as carboxypeptidase G2 (CPG2) to the tumor microenvironment, achieving efficient targeting and multifunctionality remains a significant hurdle.. This study developed a novel bifunctional fusion protein by integrating an anti-PD-L1 Jo ur na l P re -p ro of Journal Pre-proof 2 nanobody with CPG2, enabling specific immune checkpoint targeting, and localized prodrug activation.

    Article Title: Occurrence and Genetic Diversity of Trichomonas gallinae in Captive Synanthropic Birds in Southeastern Brazil.
    Article Snippet: .. PCR products were resolved via electrophoresis in a 1.0% agarose gel stained with ethidium bromide (0.5 μL/mL) in TBE buffer (pH 8.0), https://doi.org/10.3390/pathogens15040428 run at 100 V/150 mA for 50 min. A 100 bp molecular weight marker (Life Technologies®, Carlsbad, CA, USA) was used to estimate product size, visualized under a ChemiDoc MP Imaging System (Bio-Rad®, Hercules, CA, USA). ..

    Article Title: Trypanosoma minasense Parasitism in Free-Living and Captive Invasive Marmosets (Callitrichidae, Primates) in Seropédica, Rio de Janeiro, Brazil.
    Article Snippet: .. The quantity and quality of the extracted DNA were estimated on a 0.8% agarose gel using Lambda DNA (Promega®) as a molecular weight marker and via NanoDrop 2000 (Thermo Fisher Scientific®). ..

    Article Title: Integrative analysis of toxin-antitoxin systems, genomics, and phenotypic traits reveal phylogroup-dependent diversity among ExPEC bloodstream isolates.
    Article Snippet: Amplifications were performed using a VeritiTM 96-Well Thermal Cycler (Applied Biosystems). .. Following amplification, 5 μL of each PCR product was analyzed by electrophoresis on a 1.0% agarose gel stained with GelRed (Biotium, USA) in 1 × Tris-acetate-EDTA (TAE) buffer (40 mM Tris-acetate; 2 mM EDTA), at a constant voltage of 80 V. The 1 Kb Plus DNA Ladder (Invitrogen) was used as the molecular weight marker. .. Gels were visualized and documented using the Alliance 6.7 imaging system (UVItec Cambridge, UK).

    Article Title: Molecular characterization of a naturally occurring isolate of Begomovirus solanumkarnatakaense causing breakdown of Ty-3-mediated resistance in tomato plants with increased disease severity under elevated temperature.
    Article Snippet: .. M: Molecular weight marker (GeneRulerTM 100 bp plus DNA ladder, Thermo Fisher Scientific, USA); +Ve: Positive control with known plasmid DNA; H: Negative control using TNA from healthy plant; -Ve: PCR reagent control; Lane 1 to 18: PCR amplified product obtained using TNAs from 18 tomato genotypes as template in the order: ToLCD Tol-1, ToLCD Tol -2, ToLCD Tol -3, ToLCD Tol -4, ToLCD Tol -5, ToLCD Tol -6, ToLCD Tol -7, ToLCD Tol -8, ToLCD Tol -9, ToLCD Tol -10, ToLCD Tol -11, Pusa Ruby, ToLCD Tol -12, ToLCD Tol -13, ToLCD Tol -14, ToLCD Tol -15, ToLCD Tol -16, ToLCD Tol - 17. (C) Agarose gel (1%) electrophoresis of BamHI-digested RCA amplified viral DNA (lane 1). .. M: Molecular weight marker (GeneRulerTM 1 kb plus DNA ladder, Thermo Fisher Scientific, USA).

    Imaging:

    Article Title: Occurrence and Genetic Diversity of Trichomonas gallinae in Captive Synanthropic Birds in Southeastern Brazil.
    Article Snippet: .. PCR products were resolved via electrophoresis in a 1.0% agarose gel stained with ethidium bromide (0.5 μL/mL) in TBE buffer (pH 8.0), https://doi.org/10.3390/pathogens15040428 run at 100 V/150 mA for 50 min. A 100 bp molecular weight marker (Life Technologies®, Carlsbad, CA, USA) was used to estimate product size, visualized under a ChemiDoc MP Imaging System (Bio-Rad®, Hercules, CA, USA). ..

    Lambda DNA Preparation:

    Article Title: Trypanosoma minasense Parasitism in Free-Living and Captive Invasive Marmosets (Callitrichidae, Primates) in Seropédica, Rio de Janeiro, Brazil.
    Article Snippet: .. The quantity and quality of the extracted DNA were estimated on a 0.8% agarose gel using Lambda DNA (Promega®) as a molecular weight marker and via NanoDrop 2000 (Thermo Fisher Scientific®). ..

    Amplification:

    Article Title: Integrative analysis of toxin-antitoxin systems, genomics, and phenotypic traits reveal phylogroup-dependent diversity among ExPEC bloodstream isolates.
    Article Snippet: Amplifications were performed using a VeritiTM 96-Well Thermal Cycler (Applied Biosystems). .. Following amplification, 5 μL of each PCR product was analyzed by electrophoresis on a 1.0% agarose gel stained with GelRed (Biotium, USA) in 1 × Tris-acetate-EDTA (TAE) buffer (40 mM Tris-acetate; 2 mM EDTA), at a constant voltage of 80 V. The 1 Kb Plus DNA Ladder (Invitrogen) was used as the molecular weight marker. .. Gels were visualized and documented using the Alliance 6.7 imaging system (UVItec Cambridge, UK).

    Article Title: Molecular characterization of a naturally occurring isolate of Begomovirus solanumkarnatakaense causing breakdown of Ty-3-mediated resistance in tomato plants with increased disease severity under elevated temperature.
    Article Snippet: .. M: Molecular weight marker (GeneRulerTM 100 bp plus DNA ladder, Thermo Fisher Scientific, USA); +Ve: Positive control with known plasmid DNA; H: Negative control using TNA from healthy plant; -Ve: PCR reagent control; Lane 1 to 18: PCR amplified product obtained using TNAs from 18 tomato genotypes as template in the order: ToLCD Tol-1, ToLCD Tol -2, ToLCD Tol -3, ToLCD Tol -4, ToLCD Tol -5, ToLCD Tol -6, ToLCD Tol -7, ToLCD Tol -8, ToLCD Tol -9, ToLCD Tol -10, ToLCD Tol -11, Pusa Ruby, ToLCD Tol -12, ToLCD Tol -13, ToLCD Tol -14, ToLCD Tol -15, ToLCD Tol -16, ToLCD Tol - 17. (C) Agarose gel (1%) electrophoresis of BamHI-digested RCA amplified viral DNA (lane 1). .. M: Molecular weight marker (GeneRulerTM 1 kb plus DNA ladder, Thermo Fisher Scientific, USA).

    Positive Control:

    Article Title: Molecular characterization of a naturally occurring isolate of Begomovirus solanumkarnatakaense causing breakdown of Ty-3-mediated resistance in tomato plants with increased disease severity under elevated temperature.
    Article Snippet: .. M: Molecular weight marker (GeneRulerTM 100 bp plus DNA ladder, Thermo Fisher Scientific, USA); +Ve: Positive control with known plasmid DNA; H: Negative control using TNA from healthy plant; -Ve: PCR reagent control; Lane 1 to 18: PCR amplified product obtained using TNAs from 18 tomato genotypes as template in the order: ToLCD Tol-1, ToLCD Tol -2, ToLCD Tol -3, ToLCD Tol -4, ToLCD Tol -5, ToLCD Tol -6, ToLCD Tol -7, ToLCD Tol -8, ToLCD Tol -9, ToLCD Tol -10, ToLCD Tol -11, Pusa Ruby, ToLCD Tol -12, ToLCD Tol -13, ToLCD Tol -14, ToLCD Tol -15, ToLCD Tol -16, ToLCD Tol - 17. (C) Agarose gel (1%) electrophoresis of BamHI-digested RCA amplified viral DNA (lane 1). .. M: Molecular weight marker (GeneRulerTM 1 kb plus DNA ladder, Thermo Fisher Scientific, USA).

    Plasmid Preparation:

    Article Title: Molecular characterization of a naturally occurring isolate of Begomovirus solanumkarnatakaense causing breakdown of Ty-3-mediated resistance in tomato plants with increased disease severity under elevated temperature.
    Article Snippet: .. M: Molecular weight marker (GeneRulerTM 100 bp plus DNA ladder, Thermo Fisher Scientific, USA); +Ve: Positive control with known plasmid DNA; H: Negative control using TNA from healthy plant; -Ve: PCR reagent control; Lane 1 to 18: PCR amplified product obtained using TNAs from 18 tomato genotypes as template in the order: ToLCD Tol-1, ToLCD Tol -2, ToLCD Tol -3, ToLCD Tol -4, ToLCD Tol -5, ToLCD Tol -6, ToLCD Tol -7, ToLCD Tol -8, ToLCD Tol -9, ToLCD Tol -10, ToLCD Tol -11, Pusa Ruby, ToLCD Tol -12, ToLCD Tol -13, ToLCD Tol -14, ToLCD Tol -15, ToLCD Tol -16, ToLCD Tol - 17. (C) Agarose gel (1%) electrophoresis of BamHI-digested RCA amplified viral DNA (lane 1). .. M: Molecular weight marker (GeneRulerTM 1 kb plus DNA ladder, Thermo Fisher Scientific, USA).

    Negative Control:

    Article Title: Molecular characterization of a naturally occurring isolate of Begomovirus solanumkarnatakaense causing breakdown of Ty-3-mediated resistance in tomato plants with increased disease severity under elevated temperature.
    Article Snippet: .. M: Molecular weight marker (GeneRulerTM 100 bp plus DNA ladder, Thermo Fisher Scientific, USA); +Ve: Positive control with known plasmid DNA; H: Negative control using TNA from healthy plant; -Ve: PCR reagent control; Lane 1 to 18: PCR amplified product obtained using TNAs from 18 tomato genotypes as template in the order: ToLCD Tol-1, ToLCD Tol -2, ToLCD Tol -3, ToLCD Tol -4, ToLCD Tol -5, ToLCD Tol -6, ToLCD Tol -7, ToLCD Tol -8, ToLCD Tol -9, ToLCD Tol -10, ToLCD Tol -11, Pusa Ruby, ToLCD Tol -12, ToLCD Tol -13, ToLCD Tol -14, ToLCD Tol -15, ToLCD Tol -16, ToLCD Tol - 17. (C) Agarose gel (1%) electrophoresis of BamHI-digested RCA amplified viral DNA (lane 1). .. M: Molecular weight marker (GeneRulerTM 1 kb plus DNA ladder, Thermo Fisher Scientific, USA).

    Control:

    Article Title: Molecular characterization of a naturally occurring isolate of Begomovirus solanumkarnatakaense causing breakdown of Ty-3-mediated resistance in tomato plants with increased disease severity under elevated temperature.
    Article Snippet: .. M: Molecular weight marker (GeneRulerTM 100 bp plus DNA ladder, Thermo Fisher Scientific, USA); +Ve: Positive control with known plasmid DNA; H: Negative control using TNA from healthy plant; -Ve: PCR reagent control; Lane 1 to 18: PCR amplified product obtained using TNAs from 18 tomato genotypes as template in the order: ToLCD Tol-1, ToLCD Tol -2, ToLCD Tol -3, ToLCD Tol -4, ToLCD Tol -5, ToLCD Tol -6, ToLCD Tol -7, ToLCD Tol -8, ToLCD Tol -9, ToLCD Tol -10, ToLCD Tol -11, Pusa Ruby, ToLCD Tol -12, ToLCD Tol -13, ToLCD Tol -14, ToLCD Tol -15, ToLCD Tol -16, ToLCD Tol - 17. (C) Agarose gel (1%) electrophoresis of BamHI-digested RCA amplified viral DNA (lane 1). .. M: Molecular weight marker (GeneRulerTM 1 kb plus DNA ladder, Thermo Fisher Scientific, USA).



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